The Autobiography of Harkhuf is a private tomb inscription from ancient Egypt. It is significant in Egyptology as one of the two most important, and the most famous, autobiographical inscriptions of Old Kingdom officials.

His name sometimes spelled as Herkhuf, Horkhuf, or Hirkhuf, all that is known of his life comes from the inscriptions in his tomb at Qubbet el-Hawa on the west bank of the Nile at Aswan, near the First Cataract of the Nile. He was a native of Elephantine. Harkhuf served under kings Merenre I, fourth king of the 6th Dynasty (ca. 2255–2246 B.C.), and Pepi II, the last powerful king of the 6th Dynasty (ca. 2246–2152 B.C.). He was appointed governor of Upper Egypt. His primary business was trade with Nubia, forging political bonds with local leaders, and preparing the ground for an Egyptian expansion into Nubia. He led four major expeditions to Nubia. His written account of these expeditions is the most important source for Egypt's relations with Nubia at this time.

The inscriptions in Harkhuf's tomb reflect changes in the Egyptian world view that were occurring during the Late Old Kingdom and the First Intermediate Period, with the person of the king becoming more human and displaying emotions and interests, while commenting on a person leading a moral life by helping his neighbour: “I gave bread to the hungry, clothing to the naked, I ferried him who had no boat.”